This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simón, O.
Right arrow Articles by Caballero, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simón, O.
Right arrow Articles by Caballero, P.
Agricola
Right arrow Articles by Simón, O.
Right arrow Articles by Caballero, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2004, p. 5579-5588, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5579-5588.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Structure of a Spodoptera frugiperda Nucleopolyhedrovirus Population: High Prevalence of Deletion Genotypes

Oihane Simón,1 Trevor Williams,1 Miguel López-Ferber,2 and Primitivo Caballero1*

Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona, Spain,1 Laboratoire de Pathologie Comparée, UMR 5087, INRA-CNRS-Université de Montpellier II, Saint Christol-lez-Ales, France2

Received 10 January 2004/ Accepted 14 May 2004

A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant SfNIC-B was selected as the standard because its restriction profile corresponded to that of the wild-type isolate. Physical maps were generated for each of the variants. The differences between variants and the SfNIC-B standard were confined to the region between map units 9 and 32.5. This region included PstI-G, PstI-F, PstI-L, PstI-K and EcoRI-L fragments. Eight genotypes presented a deletion in their genome compared with SfNIC-B. Occlusion body-derived virions of SfNIC-C, -D and -G accounted for 41% of plaque-purified clones. These variants were not infectious per os but retained infectivity by injection into S. frugiperda larvae. Median 50% lethal concentration values for the other cloned genotypes were significantly higher than that of the wild type. The variants also differed in their speed of kill. Noninfectious variants SfNIC-C and -D lacked the pif and pif-2 genes. Infectivity was restored to these variants by plasmid rescue with a plasmid comprising both pif and pif-2. Transcription of an SfNIC-G gene was detected by reverse transcription-PCR in insects, but no fatal disease developed. Transcription was not detected in SfNIC-C or -D-inoculated larvae. We conclude that the SfNIC population presents high levels of genetic diversity, localized to a 17-kb region containing pif and pif-2, and that interactions among complete and deleted genotypic variants will likely influence the capacity of this virus to control insect pests.

* Corresponding author. Mailing address: Departamento de Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain. Phone: 34 948169129. Fax: 34 948169732. E-mail: pcm92{at}unavarra.es.

Applied and Environmental Microbiology, September 2004, p. 5579-5588, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5579-5588.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Clavijo, G., Williams, T., Simon, O., Munoz, D., Cerutti, M., Lopez-Ferber, M., Caballero, P. (2009). Mixtures of Complete and pif1- and pif2-Deficient Genotypes Are Required for Increased Potency of an Insect Nucleopolyhedrovirus. J. Virol. 83: 5127-5136 [Abstract] [Full Text]  
  • Simon, O., Williams, T., Asensio, A. C., Ros, S., Gaya, A., Caballero, P., Possee, R. D. (2008). Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies. J. Virol. 82: 7897-7904 [Abstract] [Full Text]  
  • Harrison, R. L., Puttler, B., Popham, H. J. R. (2008). Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus. J. Gen. Virol. 89: 775-790 [Abstract] [Full Text]  
  • Simon, O., Williams, T., Caballero, P., Lopez-Ferber, M. (2006). Dynamics of deletion genotypes in an experimental insect virus population. Proc R Soc B 273: 783-790 [Abstract] [Full Text]  
  • Simon, O., Williams, T., Lopez-Ferber, M., Caballero, P. (2005). Functional Importance of Deletion Mutant Genotypes in an Insect Nucleopolyhedrovirus Population. Appl. Environ. Microbiol. 71: 4254-4262 [Abstract] [Full Text]  
  • Martinez, A.-M., Williams, T., Lopez-Ferber, M., Caballero, P. (2005). Optical Brighteners Do Not Influence Covert Baculovirus Infection of Spodoptera frugiperda. Appl. Environ. Microbiol. 71: 1668-1670 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»