This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Oliveira, V. C.
Right arrow Articles by Poirier, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Oliveira, V. C.
Right arrow Articles by Poirier, Y.
Agricola
Right arrow Articles by de Oliveira, V. C.
Right arrow Articles by Poirier, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2004, p. 5685-5687, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5685-5687.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Increasing the Carbon Flux toward Synthesis of Short-Chain-Length—Medium-Chain-Length Polyhydroxyalkanoate in the Peroxisome of Saccharomyces cerevisiae through Modification of the ß-Oxidation Cycle

Valeria Cora de Oliveira,{dagger} Isamu Maeda,{ddagger} Syndie Delessert, and Yves Poirier*

Département de Biologie Moléculaire Végétale, Université de Lausanne, Lausanne, Switzerland

Received 20 April 2004/ Accepted 19 May 2004

ABSTRACT

Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the ß-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the ß-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.

* Corresponding author. Mailing address: Département de Biologie Moléculaire Végétale, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 4222. Fax: 41 21 692 4195. E-mail: yves.poirier{at}ie-bpv.unil.ch.

FOOTNOTES

{dagger} Present address: Instituto de Ciências Biomédicas II, Universidade de So Paulo, São Paulo, Brazil.

{ddagger} Present address: Laboratory of Applied Microbiology, Department of Bioproductive Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya 321-8505, Japan.

Applied and Environmental Microbiology, September 2004, p. 5685-5687, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5685-5687.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, B., Carlson, R., Srienc, F. (2006). Engineering the Monomer Composition of Polyhydroxyalkanoates Synthesized in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 72: 536-543 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»