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Applied and Environmental Microbiology, September 2004, p. 5695-5697, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5695-5697.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid Estimation of Numbers of Fecal Bacteroidetes by Use of a Quantitative PCR Assay for 16S rRNA Genes

Linda K. Dick and Katharine G. Field*

Department of Microbiology, Oregon State University, Corvallis, Oregon

Received 25 August 2003/ Accepted 14 May 2004

ABSTRACT

Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.

* Corresponding author. Mailing address: Department of Microbiology, Oregon State University, Corvallis, OR 97331. Phone: (541) 737-1837. Fax: (541) 737-0496. E-mail: kate.field{at}orst.edu.

Applied and Environmental Microbiology, September 2004, p. 5695-5697, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5695-5697.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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