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Applied and Environmental Microbiology, September 2004, p. 5704-5707, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5704-5707.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Production of Minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800

Hee-Yeon Cho,1 Hideaki Yukawa,2 Masayuki Inui,2 Roy H. Doi,1* and Sui-Lam Wong3

Section of Molecular and Cellular Biology, University of California, Davis, California,1 Research Institute of Innovative Technology for the Earth, Kyoto, Japan,2 Department of Biological Sciences, The University of Calgary, Calgary, Alberta, Canada3

Received 11 May 2004/ Accepted 22 May 2004

ABSTRACT

Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vivo synthesis of minicellulosomes suggests that it will be possible in the future to insert into B. subtilis cellulosomal genes that will allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.

* Corresponding author. Mailing address: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu.

Applied and Environmental Microbiology, September 2004, p. 5704-5707, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5704-5707.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zagorski, N. (2007). Profile of Roy H. Doi. Proc. Natl. Acad. Sci. USA 104: 9555-9557 [Full Text]  
  • Arai, T., Matsuoka, S., Cho, H.-Y., Yukawa, H., Inui, M., Wong, S.-L., Doi, R. H. (2007). Inaugural Article: Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proc. Natl. Acad. Sci. USA 104: 1456-1460 [Abstract] [Full Text]  
  • Mingardon, F., Perret, S., Belaich, A., Tardif, C., Belaich, J.-P., Fierobe, H.-P. (2005). Heterologous Production, Assembly, and Secretion of a Minicellulosome by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 71: 1215-1222 [Abstract] [Full Text]  
  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  


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