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Applied and Environmental Microbiology, January 2005, p. 282-289, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.282-289.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 Cannot Be Distinguished by Sequence Analysis of Two Surface Protein Genes and Three Housekeeping Genes

Helena Aurell,1 Pierre Farge,1 Hélène Meugnier,1 Manolo Gouy,2 Françoise Forey,1 Gérard Lina,1 François Vandenesch,1 Jerome Etienne,1 and Sophie Jarraud1*

Centre National de Référence des Legionella, INSERM E-0230, Laboratoire de Bactériologie, Faculté de Médecine Laennec IFR 62, Lyon,1 Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Claude Bernard, Villeurbanne, France2

Received 10 March 2004/ Accepted 26 August 2004

We used gene sequencing to determine whether clinical (sporadic, epidemic, and endemic) and environmental isolates of Legionella pneumophila serogroup (sg) 1 belong to specific lineages. A total of 178 clinical and environmental L. pneumophila sg 1 isolates, defined by pulsed-field gel electrophoresis and epidemiological data as sporadic, epidemic, or endemic, were analyzed for polymorphisms in five gene fragments. The fragments belonged to three housekeeping genes (coding for aconitase [acn], aspartate-ß-semialdehyde dehydrogenase [asd], and RNA polymerase ß subunit [rpoB]) and two surface protein genes (coding for the macrophage infectivity potentiator [mip] and the major outer membrane protein [mompS]). The phylogenetic tree inferred from sequence polymorphisms of the five genes identified two large clusters, one consisting of 133 poorly differentiated strains and containing two smaller clusters (10 and 2 strains) unrelated to each other and the other consisting of 42 strains. Clinical and environmental isolates could not be distinguished on this basis, and no link between genetic background and epidemiological type was found, suggesting that other factors are responsible for differences in pathogenicity.

* Corresponding author. Mailing address: Centre National de Référence des Legionella, INSERM E-0230, Laboratoire de Bactériologie, Faculté de Médecine Laennec IFR 62, 7 rue Guillaume Paradin, 69372 Lyon cedex 08, France. Phone: 33 4 78 77 86 57. Fax: 33 4 78 77 86 58. E-mail: sophie.jarraud{at}univ-lyon1.fr.

Applied and Environmental Microbiology, January 2005, p. 282-289, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.282-289.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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