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Applied and Environmental Microbiology, January 2005, p. 331-338, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.331-338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors

Dimitar Karakashev,^1,2 Damien J. Batstone,¹ and Irini Angelidaki^1*

Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark,¹ Research Group of Mathematical Modelling and Computer Sciences, Department of Microbial Biochemistry, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria²

Received 27 May 2004/ Accepted 30 August 2004

The influence of environmental parameters on the diversity of methanogenic communities in 15 full-scale biogas plants operating under different conditions with either manure or sludge as feedstock was studied. Fluorescence in situ hybridization was used to identify dominant methanogenic members of the Archaea in the reactor samples; enriched and pure cultures were used to support the in situ identification. Dominance could be identified by a positive response by more than 90% of the total members of the Archaea to a specific group- or order-level probe. There was a clear dichotomy between the manure digesters and the sludge digesters. The manure digesters contained high levels of ammonia and of volatile fatty acids (VFA) and were dominated by members of the Methanosarcinaceae, while the sludge digesters contained low levels of ammonia and of VFA and were dominated by members of the Methanosaetaceae. The methanogenic diversity was greater in reactors operating under mesophilic temperatures. The impact of the original inoculum used for the reactor start-up was also investigated by assessment of the present population in the reactor. The inoculum population appeared to have no influence on the eventual population.

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* Corresponding author. Mailing address: Environment & Resources DTU, Technical University of Denmark, Bygningstorvet, Building 115, DK-2800 Kongens Lyngby, Denmark. Phone: (45) 45251429. Fax: (45) 45932850. E-mail: ria{at}er.dtu.dk.

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Applied and Environmental Microbiology, January 2005, p. 331-338, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.331-338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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