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Applied and Environmental Microbiology, January 2005, p. 501-506, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.501-506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

Cecilia Jernberg,1,2 Åsa Sullivan,2 Charlotta Edlund,1,2 and Janet K. Jansson3*

Section for Natural Sciences, Södertörn University College, Huddinge,1 Department of Laboratory Medicine, Karolinska Institute, Stockholm,2 Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden3

Received 29 April 2004/ Accepted 29 August 2004

Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administration of clindamycin. During treatment, four subjects were given a probiotic, and four subjects were given a placebo. Changes in the microbial intestinal community composition and relative abundance of specific microbial populations in each subject were monitored by using viable counts and T-RFLP fingerprints. T-RFLP was also used to monitor specific bacterial populations that were either positively or negatively affected by clindamycin. Some dominant bacterial groups, such as Eubacterium spp., were easily monitored by T-RFLP, while they were hard to recover by cultivation. Furthermore, the two probiotic Lactobacillus strains were easily tracked by T-RFLP and were shown to be the dominant Lactobacillus community members in the intestinal microflora of subjects who received the probiotic.

* Corresponding author. Mailing address: Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, 750 07 Uppsala, Sweden. Phone: 46 18673201. Fax: 46 18673392. E-mail: janet.jansson{at}mikrob.slu.se.

Applied and Environmental Microbiology, January 2005, p. 501-506, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.501-506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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