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Applied and Environmental Microbiology, January 2005, p. 58-64, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.58-64.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effect of Culture Conditions on Microorganism Identification by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Nancy Valentine,* Sharon Wunschel, David Wunschel, Catherine Petersen, and Karen Wahl

Pacific Northwest National Laboratory, Richland, Washington

Received 20 May 2004/ Accepted 23 August 2004

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identification with MALDI-TOF MS is still possible with the protocol established at the Pacific Northwest National Laboratory (K. H. Jarman, S. T. Cebula, A. J. Saenz, C. E. Petersen, N. B. Valentine, M. T. Kingsley, and K. L. Wahl, Anal. Chem. 72:1217-1223, 2000). A core set of small proteins remain constant under at least four different culture media conditions and blood agar plates, including minimal medium M9, rich media, tryptic soy broth (TSB) or Luria-Bertani (LB) broth, and blood agar plates, such that analysis of the intact cells by matrix-assisted laser desorption/ionization mass spectrometry allows for consistent identification.

* Corresponding author. Mailing address: Pacific Northwest National Laboratory, P.O. Box 999, Battelle Blvd., K210, Richland, WA 99352. Phone: (509) 375-2070. Fax: (509) 372-4732. E-mail: nancy.valentine{at}pnl.gov.

Applied and Environmental Microbiology, January 2005, p. 58-64, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.58-64.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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