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Applied and Environmental Microbiology, January 2005, p. 580-586, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.580-586.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Resistance-Nodulation-Division Transporters from Solvent- and Drug-Resistant Bacteria in Petroleum-Contaminated Soil

Norika Meguro,1 Yumiko Kodama,1 Maria-Trinidad Gallegos,2 and Kazuya Watanabe1*

Laboratory of Applied Microbiology, Marine Biotechnology Institute, Heita, Kamaishi, Iwate, Japan,1 Department of Plant Biochemistry, Molecular and Cell Biology, Estación Experimental del Zaidín, Granada, Spain2

Received 15 February 2004/ Accepted 31 August 2004

PCR assays for analyzing resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in soil were developed. Sequence analysis of amplicons showed that the PCR successfully retrieved transporter gene fragments from soil. Most of the genes retrieved from petroleum-contaminated soils formed a cluster (cluster PCS) that was distantly related to known transporter genes. Competitive PCR showed that the abundance of PCS genes is increased in petroleum-contaminated soil.

* Corresponding author. Mailing address: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan. Phone: 81-193-26-5781. Fax: 81-193-26-6592. E-mail: kazuya.watanabe{at}mbio.jp.

Applied and Environmental Microbiology, January 2005, p. 580-586, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.580-586.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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