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Applied and Environmental Microbiology, January 2005, p. 591-593, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.591-593.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

High-Level Overproduction of His-Tagged Tth DNA Polymerase in Thermus thermophilus

Renata Moreno,1 Amparo Haro,2 Angel Castellanos,2 and José Berenguer1*

Centro de Biología Molecular "Severo Ochoa" Consejo Superior de Investigaciones Científicas— Universidad Autónoma de Madrid, Campus de Cantoblanco,1 Biotools B & M Labs, Madrid, Spain2

Received 15 June 2004/ Accepted 24 August 2004

A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa" CSIC-UAM, Campus de Cantoblanco, 28049 Madrid, Spain. Phone: 34914978099. Fax: 34914978087. E-mail: jberenguer{at}cbm.uam.es.

Applied and Environmental Microbiology, January 2005, p. 591-593, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.591-593.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nesper, J., Brosig, A., Ringler, P., Patel, G. J., Muller, S. A., Kleinschmidt, J. H., Boos, W., Diederichs, K., Welte, W. (2008). Omp85Tt from Thermus thermophilus HB27: an Ancestral Type of the Omp85 Protein Family. J. Bacteriol. 190: 4568-4575 [Abstract] [Full Text]  


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