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Applied and Environmental Microbiology, October 2005, p. 6241-6246, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6241-6246.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Trichoderma Mitogen-Activated Protein Kinase Signaling Is Involved in Induction of Plant Systemic Resistance

Ada Viterbo,1* Michal Harel,1 Benjamin A. Horwitz,2 Ilan Chet,1 and Prasun K. Mukherjee3

Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel,1 Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel,2 Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Mumbai 400085, India3

Received 29 December 2004/ Accepted 25 April 2005

The role of a mitogen-activated protein kinase (MAPK) TmkA in inducing systemic resistance in cucumber against the bacterial pathogen Pseudomonas syringae pv. lacrymans was investigated by using tmkA loss-of-function mutants of Trichoderma virens. In an assay where Trichoderma spores were germinated in proximity to cucumber roots, the mutants were able to colonize the plant roots as effectively as the wild-type strain but failed to induce full systemic resistance against the leaf pathogen. Interactions with the plant roots enhanced the level of tmkA transcript in T. virens and its homologue in Trichoderma asperellum. At the protein level, we could detect the activation of two forms reacting to the phospho-p44/42 MAPK antibody. Biocontrol experiments demonstrated that the tmkA mutants retain their biocontrol potential against Rhizoctonia solani in soil but are not effective against Sclerotium rolfsii in reducing disease incidence. Our results show that, unlike in many plant-pathogen interactions, Trichoderma TmkA MAPK is not involved in limited root colonization. Trichoderma, however, needs MAPK signaling in order to induce full systemic resistance in the plant.

* Corresponding author. Mailing address: Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel. Phone: 972-8-9344466. Fax: 972-8-9344112. E-mail: ada.viterbo{at}weizmann.ac.il.

Applied and Environmental Microbiology, October 2005, p. 6241-6246, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6241-6246.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brotman, Y., Briff, E., Viterbo, A., Chet, I. (2008). Role of Swollenin, an Expansin-Like Protein from Trichoderma, in Plant Root Colonization. Plant Physiol. 147: 779-789 [Abstract] [Full Text]  
  • Djonovic, S., Vargas, W. A., Kolomiets, M. V., Horndeski, M., Wiest, A., Kenerley, C. M. (2007). A Proteinaceous Elicitor Sm1 from the Beneficial Fungus Trichoderma virens Is Required for Induced Systemic Resistance in Maize. Plant Physiol. 145: 875-889 [Abstract] [Full Text]  
  • Mukherjee, M., Mukherjee, P. K., Kale, S. P. (2007). cAMP signalling is involved in growth, germination, mycoparasitism and secondary metabolism in Trichoderma virens. Microbiology 153: 1734-1742 [Abstract] [Full Text]  


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