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Applied and Environmental Microbiology, October 2005, p. 6388-6389, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6388-6389.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Simple Colorimetric Method for Detecting Degenerate Strains of the Cultivated Basidiomycete Flammulina velutipes (Enokitake)

Yumi Magae,1* Kobun Akahane,2 Kimiyoshi Nakamura,2 and Shigeyuki Tsunoda2

Department of Applied Microbiology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan,1 Nagano Vegetable and Ornamental Experiment Station, Matsushiro 2206, Nagano 381-1211, Japan2

Received 16 December 2004/ Accepted 28 April 2005

Degeneration of cultivated strains of Flammulina velutipes is a serious problem. We developed a simple colorimetric method to detect degenerate strains by using a liquid medium supplemented with bromothymol blue and lactose. The ability of a strain to develop normal mushrooms could be determined by the color of the medium.

* Corresponding author. Mailing address: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan. Phone: 81-298-733211. Fax: 81-298-743720. E-mail: ymagae{at}ffpri.affrc.go.jp.

Applied and Environmental Microbiology, October 2005, p. 6388-6389, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6388-6389.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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