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Applied and Environmental Microbiology, October 2005, p. 6401-6406, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6401-6406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Sau-PCR, a Novel Amplification Technique for Genetic Fingerprinting of Microorganisms{dagger}

Viviana Corich, Alessandro Mattiazzi, Elisa Soldati, Angela Carraro, and Alessio Giacomini*

Dipartimento di Biotecnologie Agrarie, Università di Padova, Legnaro, Padova, Italy

Received 12 January 2005/ Accepted 16 May 2005

The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.

* Corresponding author. Mailing address: Dipartimento di Biotecnologie Agrarie, Università di Padova, viale dell'Università 16, I-35020 Legnaro, Padova, Italy. Phone: 39 049 8272924. Fax: 39 049 8272929. E-mail: alessio.giacomini{at}unipd.it.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, October 2005, p. 6401-6406, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6401-6406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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