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Applied and Environmental Microbiology, October 2005, p. 6443-6445, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6443-6445.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Production of Ethanol from Starch by Respiration-Deficient Recombinant Saccharomyces cerevisiae

Ebru Toksoy Öner,1* Stephen G. Oliver,2 and Betül Kirdar3

Department of Chemical Engineering, Marmara University, Goztepe 34722 Istanbul, Turkey,1 School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom,2 Department of Chemical Engineering, Bogaziçi University, Bebek 80815 Istanbul, Turkey3

Received 25 November 2004/ Accepted 4 June 2005

A 100%-respiration-deficient nuclear petite amylolytic Saccharomyces cerevisiae NPB-G strain was generated, and its employment for direct fermentation of starch into ethanol was investigated. In a comparison of ethanol fermentation performances with the parental respiration-sufficient WTPB-G strain, the NPB-G strain showed an increase of ca. 48% in both ethanol yield and ethanol productivity.

* Corresponding author. Mailing address: Marmara University, Facul- ty of Engineering, Department of Chemical Engineering, Goztepe 34722 Istanbul, Turkey. Phone: (90) 216 348 0292, ext. 726. Fax: (90) 216 348 0293. E-mail: etoksoy{at}eng.marmara.edu.tr.

Applied and Environmental Microbiology, October 2005, p. 6443-6445, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6443-6445.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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