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Applied and Environmental Microbiology, November 2005, p. 6473-6478, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6473-6478.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Diversity of "Candidatus Liberibacter asiaticus," Based on the omp Gene Sequence

C. Bastianel, M. Garnier-Semancik, J. Renaudin, J. M. Bové, and S. Eveillard*

Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, INRA, Université Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP81, 33883 Villenave D'Ornon Cedex, France

Received 17 December 2004/ Accepted 6 June 2005

Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species "Ca. Liberibacter asiaticus," even within a given region, may comprise several different variants. Thus, omp-based PCR-RFLP analysis is a simple method for detecting and differentiating "Ca. Liberibacter asiaticus" isolates.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, INRA, Université Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP81, 33883 Villenave D'Ornon Cedex, France. Phone: (33) 557 12 23 63. Fax: (33) 557 12 23 69. E-mail: jagoueix{at}bordeaux.inra.fr.

Applied and Environmental Microbiology, November 2005, p. 6473-6478, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6473-6478.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wulff, N. A., Eveillard, S., Foissac, X., Ayres, A. J., Bove, J.-M. (2009). rRNA operons and genome size of 'Candidatus Liberibacter americanus', a bacterium associated with citrus huanglongbing in Brazil. Int. J. Syst. Evol. Microbiol. 59: 1984-1991 [Abstract] [Full Text]  


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