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Applied and Environmental Microbiology, November 2005, p. 6698-6701, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6698-6701.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Intervening Sequence Acquired by Lateral Gene Transfer in Tropheryma whipplei Results in 23S rRNA Fragmentation

Nicolas Crapoulet, Sylvianne Robineau, Didier Raoult, and Patricia Renesto*

Unité des Rickettsies, CNRS-UMR6020, IFR48, Faculté de Médecine, 27, Bd. Jean Moulin, Marseille, France

Received 23 February 2005/ Accepted 19 July 2005

Completion of Tropheryma whipplei genome sequencing may provide insights into the evolution of the molecular mechanisms underlying the pathogenicity of this microorganism. The first postgenomic application was the successful design of a comprehensive culture medium that allows axenic growth of this bacterium, which is particularly recalcitrant to cultivation. This achievement in turn permitted analysis of T. whipplei RNA without contaminating eukaryotic nucleic acids. To obtain high-quality RNA, several extraction methods were compared, but under all conditions tested an atypical profile was observed. By using a Northern blot assay we demonstrated that an insertion sequence previously described in T. whipplei 23S rRNA is in fact an intervening sequence excised during maturation. This cleavage could involve an RNase III identified in the genome of this microorganism. Among the bacteria with a 23S rRNA insertion sequence, T. whipplei is the only gram-positive microorganism. We present phylogenetic evidence that this mobile genetic element was acquired by lateral gene transfer from another enteric bacterium.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR6020, IFR48, Faculté de Médecine, 27, Boulevard Jean Moulin, 13385 Marseille, France. Phone: (33) 491 32 43 75. Fax: (33) 491 38 77 72. E-mail: patricia.renesto{at}medecine.univ-mrs.fr.

Applied and Environmental Microbiology, November 2005, p. 6698-6701, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6698-6701.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Van La, M., Barbry, P., Raoult, D., Renesto, P. (2007). Molecular basis of Tropheryma whipplei doxycycline susceptibility examined by transcriptional profiling. J Antimicrob Chemother 59: 370-377 [Abstract] [Full Text]  
  • Crapoulet, N., Barbry, P., Raoult, D., Renesto, P. (2006). Global Transcriptome Analysis of Tropheryma whipplei in Response to Temperature Stresses.. J. Bacteriol. 188: 5228-5239 [Abstract] [Full Text]  


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