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Applied and Environmental Microbiology, November 2005, p. 6769-6775, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6769-6775.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Expression of Rumen Microbial Fibrolytic Enzyme Genes in Probiotic Lactobacillus reuteri

Je-Ruei Liu,1,3 Bi Yu,2 Fu-Hwa Liu,1 Kuo-Joan Cheng,1 and Xin Zhao3*

Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan,1 Department of Animal Science, College of Agriculture and Natural Resources, National Chung-Hsing University, Taichung, Taiwan,2 Department of Animal Science, Faculty of Agricultural and Environmental Sciences, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada3

Received 19 January 2005/ Accepted 9 June 2005

This study was aimed at evaluating the cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of Lactobacillus reuteri and investigating the probiotic characteristics of these genetically modified lactobacilli. The Neocallimastix patriciarum xylanase gene xynCDBFV, the Fibrobacter succinogenes ß-glucanase (1,3-1,4-ß-D-glucan 4-glucanohydrolase [EC 3.2.1.73]) gene, and the Piromyces rhizinflata cellulase gene eglA were cloned in a strain of L. reuteri isolated from the gastrointestinal tract of broilers. The enzymes were expressed and secreted under the control of the Lactococcus lactis lacA promoter and its secretion signal. The L. reuteri transformed strains not only acquired the capacity to break down soluble carboxymethyl cellulose, ß-glucan, or xylan but also showed high adhesion efficiency to mucin and mucus and resistance to bile salt and acid.

* Corresponding author. Mailing address: Department of Animal Science, McGill University, 21111 Lakeshore Rd., Ste-Anne-de-Bellevue, Quebec, Canada H9X3V9. Phone: (514) 398-7975. Fax: (514) 398-7964. E-mail: Xin.Zhao{at}mcgill.ca.

Applied and Environmental Microbiology, November 2005, p. 6769-6775, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6769-6775.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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