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Applied and Environmental Microbiology, November 2005, p. 7292-7300, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7292-7300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms

Salme Timmusk,* Nina Grantcharova, and E. Gerhart H. Wagner

Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, S-751 24 Uppsala, Sweden

Received 25 March 2005/ Accepted 28 June 2005

Paenibacillus polymyxa is a plant growth-promoting rhizobacterium with a broad host range, but so far the use of this organism as a biocontrol agent has not been very efficient. In previous work we showed that this bacterium protects Arabidopsis thaliana against pathogens and abiotic stress (S. Timmusk and E. G. H. Wagner, Mol. Plant-Microbe Interact. 12:951-959, 1999; S. Timmusk, P. van West, N. A. R. Gow, and E. G. H. Wagner, p. 1-28, in Mechanism of action of the plant growth promoting bacterium Paenibacillus polymyxa, 2003). Here, we studied colonization of plant roots by a natural isolate of P. polymyxa which had been tagged with a plasmid-borne gfp gene. Fluorescence microscopy and electron scanning microscopy indicated that the bacteria colonized predominantly the root tip, where they formed biofilms. Accumulation of bacteria was observed in the intercellular spaces outside the vascular cylinder. Systemic spreading did not occur, as indicated by the absence of bacteria in aerial tissues. Studies were performed in both a gnotobiotic system and a soil system. The fact that similar observations were made in both systems suggests that colonization by this bacterium can be studied in a more defined system. Problems associated with green fluorescent protein tagging of natural isolates and deleterious effects of the plant growth-promoting bacteria are discussed.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, Box 596, S-751 24 Uppsala, Sweden. Phone: 46 73 70154 02. Fax: 46 18 530396. E-mail: salme.timmusk{at}evp.slu.se.

Applied and Environmental Microbiology, November 2005, p. 7292-7300, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7292-7300.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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