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Applied and Environmental Microbiology, November 2005, p. 7528-7530, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7528-7530.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

Trine Danø Klingberg,1* Maja Herold Pedersen,1 Avrelija Cencic,2 and Birgitte Bjørn Budde1

Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark,1 Faculty of Agriculture, University of Maribor, Maribor, Slovenia2

Received 8 April 2005/ Accepted 5 July 2005

Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes.

* Corresponding author. Mailing address: The Royal Veterinary and Agricultural University, Department of Food Science, Rolighedsvej 30, 4th Floor, DK-1958 Frederiksberg C, Denmark. Phone: 45 35 28 33 26. Fax: 45 35 28 32 14. E-mail: tdk{at}kvl.dk.

Applied and Environmental Microbiology, November 2005, p. 7528-7530, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7528-7530.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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