This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishida, Y.
Right arrow Articles by Ohtani, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishida, Y.
Right arrow Articles by Ohtani, H.
Agricola
Right arrow Articles by Ishida, Y.
Right arrow Articles by Ohtani, H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2005, p. 7539-7541, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7539-7541.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

On-Probe Sample Pretreatment for Direct Analysis of Lipids in Gram-Positive Bacterial Cells by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Yasuyuki Ishida,1* Kuniyuki Kitagawa,1 Akihito Nakayama,1 and Hajime Ohtani2

Division of Energy Science, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan,1 Department of Material Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan2

Received 31 January 2005/ Accepted 6 July 2005

On-probe sample pretreatment using trifluoroacetic acid as an additional reagent enabled the direct detection of phospholipids in whole bacteria by means of matrix-assisted laser desorption ionization mass spectrometry for not only gram-negative organisms but also gram-positive ones with a thicker peptidoglycan layer.

* Corresponding author. Mailing address: Division of Energy Science, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. Phone: (81) 52-789-5479. Fax: (81) 52-789-5479. E-mail: ishida{at}esi.nagoya-u.ac.jp.

Applied and Environmental Microbiology, November 2005, p. 7539-7541, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7539-7541.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»