This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freeman, J. L.
Right arrow Articles by Salt, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freeman, J. L.
Right arrow Articles by Salt, D. E.
Agricola
Right arrow Articles by Freeman, J. L.
Right arrow Articles by Salt, D. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2005, p. 8627-8633, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8627-8633.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nickel and Cobalt Resistance Engineered in Escherichia coli by Overexpression of Serine Acetyltransferase from the Nickel Hyperaccumulator Plant Thlaspi goesingense

John L. Freeman,{dagger} Michael W. Persans,{ddagger} Ken Nieman, and David E. Salt*

Center for Plant Environmental Stress Physiology, Purdue University, 625 Agricultural Mall Dr., West Lafayette, Indiana 47907

Received 23 May 2005/ Accepted 8 September 2005

The overexpression of serine acetyltransferase from the Ni-hyperaccumulating plant Thlaspi goesingense causes enhanced nickel and cobalt resistance in Escherichia coli. Furthermore, overexpression of T. goesingense serine acetyltransferase results in enhanced sensitivity to cadmium and has no significant effect on resistance to zinc. Enhanced nickel resistance is directly related to the constitutive overactivation of sulfur assimilation and glutathione biosynthesis, driven by the overproduction of O-acetyl-L-serine, the product of serine acetyltransferase and a positive regulator of the cysteine regulon. Nickel in the serine acetyltransferase-overexpressing strains is not detoxified by coordination or precipitation with sulfur, suggesting that glutathione is involved in reducing the oxidative damage imposed by nickel.

* Corresponding author. Mailing address: Center for Plant Environmental Stress Physiology, Purdue University, 625 Agricultural Mall Drive, West Lafayette, IN 47907. Phone: (765) 496-2112. Fax: (765) 494-0391. E-mail: dsalt{at}purdue.edu.

{dagger} Present address: Department of Biology, Colorado State University, Fort Collins, CO 80523.

{ddagger} Present address: Department of Biology, University of Texas—Pan American, Edinburg, TX 78541.

Applied and Environmental Microbiology, December 2005, p. 8627-8633, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8627-8633.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Thorgersen, M. P., Downs, D. M. (2009). Oxidative stress and disruption of labile iron generate specific auxotrophic requirements in Salmonella enterica. Microbiology 155: 295-304 [Abstract] [Full Text]  
  • Thorgersen, M. P., Downs, D. M. (2007). Cobalt Targets Multiple Metabolic Processes in Salmonella enterica. J. Bacteriol. 189: 7774-7781 [Abstract] [Full Text]  
  • Mirete, S., de Figueras, C. G., Gonzalez-Pastor, J. E. (2007). Novel Nickel Resistance Genes from the Rhizosphere Metagenome of Plants Adapted to Acid Mine Drainage. Appl. Environ. Microbiol. 73: 6001-6011 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»