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Applied and Environmental Microbiology, December 2005, p. 8895-8902, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8895-8902.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cell Adsorption and Selective Desorption for Separation of Microbial Cells by Using Chitosan-Immobilized Silica

Munehiro Kubota,1 Masayoshi Matsui,2 Hiroyuki Chiku,1 Nobuyuki Kasashima,1 Manabu Shimojoh,3 and Kengo Sakaguchi1*

Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan,1 Nanoarchitectonics Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8565, Japan,2 Research and Development Department, Toyo Suisan Kaisha, Ltd., Minato, Tokyo 108-8501, Japan3

Received 10 March 2005/ Accepted 10 August 2005

Cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (CIS). When chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. Testing of the adsorption and desorption ability of CIS using various microbes such as Escherichia coli, Aeromonas hydrophila, Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus casei, Streptococcus mutans, Streptococcus sobrinus, Streptococcus salivarius, Saccharomyces cerevisiae, Saccharomyces ludwigii, and Schizosaccharomyces pombe revealed that most microbes could be adsorbed and selectively desorbed under different conditions. In particular, recovery was improved when L-cysteine was added. A mixture of two bacterial strains adsorbed onto CIS could also be successfully separated by use of specific solutions for each strain. Most of the desorbed cells were alive. Thus, quantitative and selective fractionation of cells is readily achievable by employing chitosan, a known antibacterial material.

* Corresponding author. Mailing address: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamzaki, Noda-shi, Chiba-ken 278-8510, Japan. Phone: 81-4-7124-1501, ext. 3409. Fax: 81-4-7123-9767. E-mail: kengo{at}rs.noda.tus.ac.jp.

Applied and Environmental Microbiology, December 2005, p. 8895-8902, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8895-8902.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kubota, M., Hayashi, T., Iwasaki, K., Ohtsuka, H., Kohiruimaki, M., Kawamura, S., Sakaguchi, K., Abe, R. (2007). Rapid and Effective Method for Separation of Staphylococcus aureus from Somatic Cells in Mastitis Milk. J DAIRY SCI 90: 4100-4107 [Abstract] [Full Text]  


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