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Applied and Environmental Microbiology, December 2005, p. 8920-8924, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8920-8924.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Plasmids for Nicotine-Dependent and -Independent Gene Expression in Arthrobacter nicotinovorans and Other Arthrobacter Species

Cristinel Sandu,* Calin-Bogdan Chiribau, Paula Sachelaru, and Roderich Brandsch

Institute of Biochemistry and Molecular Biology, University of Freiburg, Hermann Herder Str. 7, 79104 Freiburg, Germany

Received 23 June 2005/ Accepted 3 September 2005

The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.

* Corresponding author. Present address: Laboratory of Molecular Biophysics, The Rockefeller University, 1230 York Avenue, Box 42, New York, NY 10021. Phone: (212) 327-7220. Fax: (219) 327-7222. E-mail: sanduc{at}rockefeller.edu.

Applied and Environmental Microbiology, December 2005, p. 8920-8924, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8920-8924.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ganas, P., Brandsch, R. (2009). Uptake of L-nicotine and of 6-hydroxy-L-nicotine by Arthrobacter nicotinovorans and by Escherichia coli is mediated by facilitated diffusion and not by passive diffusion or active transport. Microbiology 155: 1866-1877 [Abstract] [Full Text]  
  • Ganas, P., Mihasan, M., Igloi, G. L., Brandsch, R. (2007). A two-component small multidrug resistance pump functions as a metabolic valve during nicotine catabolism by Arthrobacter nicotinovorans. Microbiology 153: 1546-1555 [Abstract] [Full Text]  
  • Sachelaru, P., Schiltz, E., Brandsch, R. (2006). A Functional mobA Gene for Molybdopterin Cytosine Dinucleotide Cofactor Biosynthesis Is Required for Activity and Holoenzyme Assembly of the Heterotrimeric Nicotine Dehydrogenases of Arthrobacter nicotinovorans.. Appl. Environ. Microbiol. 72: 5126-5131 [Abstract] [Full Text]  


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