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Applied and Environmental Microbiology, December 2005, p. 8937-8940, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8937-8940.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Improvement of Catalytic Properties of Escherichia coli Penicillin G Acylase Immobilized on Glyoxyl Agarose by Addition of a Six-Amino-Acid Tag

Francesca Scaramozzino,1,§ Ilona Estruch,2 Paola Rossolillo,1 Marco Terreni,2 and Alessandra M. Albertini1*

Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Ferrata 1, 27100 Pavia, Italy,1 Laboratorio di Farmaceutica e Biocatalisi, Dipartimento di Chimica Farmaceutica, Università degli Studi di Pavia, Via Taramelli 12, 27100 Pavia, Italy2

Received 10 January 2005/ Accepted 31 July 2005

A tag of three lysines alternating with three glycines was added to the C-terminal end of the ß chain of penicillin G acylase (PGA). This modification improved the immobilization efficiency of PGA on glyoxyl agarose and the catalytic properties of the PGA derivative, although it impaired the posttranslational steps of overexpressed protein maturation.

* Corresponding author. Mailing address: Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Via Ferrata 1, 27100 Pavia, Italy. Phone: (39) 0382-985549. Fax: (39) 0382-528496. E-mail: albert{at}ipvgen.unipv.it.

§ Present address: Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Calif.

Applied and Environmental Microbiology, December 2005, p. 8937-8940, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8937-8940.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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