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Applied and Environmental Microbiology, December 2005, p. 8991-8994, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8991-8994.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Forensic Profiling of Cryptosporidium Species and Genotypes in Raw Water

Norma J. Ruecker,1 Niravanh Bounsombath,1 Peter Wallis,2 Corinne S. L. Ong,3,4 Judith L. Isaac-Renton,3,4 and Norman F. Neumann1,5*

Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada,1 Hyperion Ltd., Medicine Hat, Alberta, Canada,2 British Columbia Center for Disease Control, Vancouver, British Columbia, Canada,3 Department of Laboratory Medicine and Pathology, University of British Columbia, Vancouver, British Columbia, Canada,4 Alberta Provincial Laboratory for Public Health (Microbiology), Calgary, Alberta, Canada5

Received 15 April 2005/ Accepted 22 August 2005

The emerging concept of host specificity of Cryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes of Cryptosporidium in a single water sample.

* Corresponding author. Mailing address: Environmental Microbiology, Alberta Provincial Laboratory for Public Health (Microbiology), 3030 Hospital Drive NW, Calgary, Alberta, Canada T2N 4W4. Phone: (403) 944-1236. Fax: (403) 283-0142. E-mail: n.neumann{at}provlab.ab.ca.

Applied and Environmental Microbiology, December 2005, p. 8991-8994, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8991-8994.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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