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Applied and Environmental Microbiology, December 2005, p. 8998-9007, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8998-9007.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Characterization of the Bifidobacterium breve UCC 2003 hrcA Locus

Marco Ventura,1,2* Carlos Canchaya,1 Valentina Bernini,2 Antonio Del Casale,3 Franco Dellaglio,3 Erasmo Neviani,2 Gerald F. Fitzgerald,1 and Douwe van Sinderen1

Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland,1 Department of Genetic, Anthropology and Evolution, University of Parma, Parma, Italy,2 Dipartimento Scientifico e Tecnologico, University of Verona, Verona, Italy3

Received 28 July 2005/ Accepted 14 September 2005

The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes and transcriptional regulators, including the DnaJ and the HrcA proteins. Genome analysis of Bifidobacterium breve UCC 2003 revealed a second copy of a dnaJ gene, named dnaJ2, which is flanked by the hrcA gene in a genetic constellation that appears to be unique to the actinobacteria. Phylogenetic analysis using 53 bacterial dnaJ sequences, including both dnaJ1 and dnaJ2 sequences, suggests that these genes have followed a different evolutionary development. Furthermore, the B. breve UCC 2003 dnaJ2 gene seems to be regulated in a manner that is different from that of the previously characterized dnaJ1 gene. The dnaJ2 gene, which was shown to be part of a 2.3-kb bicistronic operon with hrcA, was induced by osmotic shock but not significantly by heat stress. This induction pattern is unlike those of other characterized dnaJ genes and may be indicative of a unique stress adaptation strategy by this commensal microorganism.

* Corresponding author. Present address: Department of Genetics, Evolution, and Anthropology, University of Parma, Parma, Italy. Phone: 39 0521 905479. Fax: 39 0521 905604. E-mail: marco.ventura{at}unipr.it.

Applied and Environmental Microbiology, December 2005, p. 8998-9007, Vol. 71, No. 12
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.12.8998-9007.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zomer, A., Fernandez, M., Kearney, B., Fitzgerald, G. F., Ventura, M., van Sinderen, D. (2009). An Interactive Regulatory Network Controls Stress Response in Bifidobacterium breve UCC2003. J. Bacteriol. 191: 7039-7049 [Abstract] [Full Text]  
  • Alexandre, A., Laranjo, M., Young, J. P. W., Oliveira, S. (2008). dnaJ is a useful phylogenetic marker for alphaproteobacteria. Int. J. Syst. Evol. Microbiol. 58: 2839-2849 [Abstract] [Full Text]  
  • Sheehan, V. M., Sleator, R. D., Hill, C., Fitzgerald, G. F. (2007). Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. Microbiology 153: 3563-3571 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Zhang, Z., Fitzgerald, G. F., van Sinderen, D. (2007). Molecular Characterization of hsp20, Encoding a Small Heat Shock Protein of Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 73: 4695-4703 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Casale, A. D., Dellaglio, F., Neviani, E., Fitzgerald, G. F., van Sinderen, D. (2006). Analysis of bifidobacterial evolution using a multilocus approach. Int. J. Syst. Evol. Microbiol. 56: 2783-2792 [Abstract] [Full Text]  


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