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Applied and Environmental Microbiology, February 2005, p. 1089-1092, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1089-1092.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Surface Plasmon Resonance Imaging-Based Protein Array Chip System for Monitoring a Hexahistidine-Tagged Protein during Expression and Purification

Hyeon-Su Ro,1,{dagger} Sun Ok Jung,1 Byung Hoon Kho,1 Hyung Pyo Hong,1 Jae Sung Lee,1 Yong-Beom Shin,1 Min Gon Kim,1 and Bong Hyun Chung1*

BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Korea1

Received 18 March 2004/ Accepted 13 September 2004

ABSTRACT

A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

* Corresponding author. Mailing address: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea. Phone: 82-42-860-4442. Fax: 82-42-860-4594. E-mail: chungbh{at}kribb.re.kr.

FOOTNOTES

{dagger} Present address: Department of Microbiology, GyeongSang National University, Chinju, GyeongNam 660-701, Korea.

Applied and Environmental Microbiology, February 2005, p. 1089-1092, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1089-1092.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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