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Applied and Environmental Microbiology, February 2005, p. 1101-1104, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1101-1104.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Purification and cDNA Cloning of NADPH-Dependent Aldoketoreductase, Involved in Asymmetric Reduction of Methyl 4-Bromo-3-Oxobutyrate, from Penicillium citrinum IFO4631

Hiroyuki Asako,1* Ryuhei Wakita,1 Kenji Matsumura,2 Masatoshi Shimizu,1 Jun Sakai,3 and Nobuya Itoh4

Organic Synthesis Research Laboratory, Sumitomo Chemical Co., Ltd.,1 Genome Science Laboratories, Sumitomo Pharmaceuticals Co., Ltd., Konohana-ku, Osaka,3 Agricultural Research Laboratory, Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo,2 Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan4

Received 16 July 2004/ Accepted 18 September 2004

ABSTRACT

Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate [(S)-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system.

* Corresponding author: Mailing address: Organic Synthesis Research Laboratory, Sumitomo Chemical Co., Ltd., 1-98 Kasugade-naka 3-chome, Konohana-ku, Osaka 554-8558, Japan. Phone: 81 6 6466 5394. Fax: 81 6 6466 5429. E-mail: asako{at}sc.sumitomo-chem.co.jp.

Applied and Environmental Microbiology, February 2005, p. 1101-1104, Vol. 71, No. 2
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.2.1101-1104.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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