This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matulova, M.
Right arrow Articles by Delort, A.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matulova, M.
Right arrow Articles by Delort, A.-M.
Agricola
Right arrow Articles by Matulova, M.
Right arrow Articles by Delort, A.-M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2005, p. 1247-1253, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1247-1253.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Degradation of Wheat Straw by Fibrobacter succinogenes S85: a Liquid- and Solid-State Nuclear Magnetic Resonance Study

M. Matulova,1,2 R. Nouaille,1,3 P. Capek,2 M. Péan,4 E. Forano,3* and A.-M. Delort1*

Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 Université Blaise Pascal-CNRS, Aubière,1 Unité de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, Saint-Genès-Champanelle,3 DEVM/GRAP, CEA Cadarache, St. Paul lez Durance, France,4 Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic2

Received 30 June 2004/ Accepted 4 October 2004

Wheat straw degradation by Fibrobacter succinogenes was monitored by nuclear magnetic resonance (NMR) spectroscopy and chemolytic methods to investigate the activity of an entire fibrolytic system on an intact complex substrate. In situ solid-state NMR with 13C cross-polarization magic angle spinning was used to monitor the modification of the composition and structure of lignocellulosic fibers (of 13C-enriched wheat straw) during the growth of bacteria on this substrate. There was no preferential degradation either of amorphous regions of cellulose versus crystalline regions or of cellulose versus hemicelluloses in wheat straw. This suggests either a simultaneous degradation of the amorphous and crystalline parts of cellulose and of cellulose and hemicelluloses by the enzymes or degradation at the surface at a molecular scale that cannot be detected by NMR. Liquid-state two-dimensional NMR experiments and chemolytic methods were used to analyze in detail the various sugars released into the culture medium. An integration of NMR signals enabled the quantification of oligosaccharides produced from wheat straw at various times of culture and showed the sequential activities of some of the fibrolytic enzymes of F. succinogenes S85 on wheat straw. In particular, acetylxylan esterase appeared to be more active than arabinofuranosidase, which was more active than {alpha}-glucuronidase. Finally, cellodextrins did not accumulate to a great extent in the culture medium.

* Corresponding author. Mailing address for A.-M. Delort: Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 Université Blaise Pascal-CNRS, 63177 Aubière, France. Phone: 33 473 40 77 14. Fax: 33 473 40 77 17. E-mail: amdelort{at}chimtp.univ-bpclermont.fr. Mailing address for E. Forano: Unité de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France. Phone: 33 473 62 42 48. Fax: 33 473 62 45 81. E-mail: forano{at}clermont.inra.fr.

Applied and Environmental Microbiology, March 2005, p. 1247-1253, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1247-1253.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chassard, C., Delmas, E., Lawson, P. A., Bernalier-Donadille, A. (2008). Bacteroides xylanisolvens sp. nov., a xylan-degrading bacterium isolated from human faeces. Int. J. Syst. Evol. Microbiol. 58: 1008-1013 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»