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Applied and Environmental Microbiology, March 2005, p. 1642-1647, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1642-1647.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa{dagger}

Ryan Woodyer,1 Michael Simurdiak,2 Wilfred A. van der Donk,1* and Huimin Zhao1,2*

Departments of Chemistry,1 Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois2

Received 18 June 2004/ Accepted 11 October 2004

ABSTRACT

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

* Corresponding author. Mailing address for Huimin Zhao: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801. Phone: (217) 333-2631. Fax: (217) 333-5052. E-mail: zhao5{at}uiuc.edu. Mailing address for Wilfred A. van der Donk: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801. Phone: (217) 244-5360. Fax: (217) 244-8024. E-mail: vddonk{at}uiuc.edu.

FOOTNOTES

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2005, p. 1642-1647, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1642-1647.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shao, Z., Zhao, H., Zhao, H. (2009). DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res 37: e16-e16 [Abstract] [Full Text]  
  • Nair, N., Zhao, H. (2007). Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa. Appl. Environ. Microbiol. 73: 2001-2004 [Abstract] [Full Text]  


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