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Applied and Environmental Microbiology, March 2005, p. 1662-1663, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1662-1663.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Probiotic Bacteria May Become Dormant during Storage

Sampo J. Lahtinen,* Miguel Gueimonde, Arthur C. Ouwehand, Johanna P. Reinikainen, and Seppo J. Salminen

Functional Foods Forum, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland

Received 30 March 2004/ Accepted 11 October 2004

ABSTRACT

The determination of bacterial viability in probiotic products is of economic, technological, and clinical significance. We compared four methods to enumerate three Bifidobacterium strains in fermented oat products during storage. A subpopulation of nonculturable cells retained a functional cell membrane typical of viable cells, indicating that probiotic bacteria become dormant during storage.

* Corresponding author. Mailing address: Functional Foods Forum/University of Turku, Itäinen pitkäkatu 4, 20014 Turku, Finland. Phone: 358 (2) 3336823. Fax: 358 (2) 3336862. E-mail: sajola{at}utu.fi.

Applied and Environmental Microbiology, March 2005, p. 1662-1663, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1662-1663.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lahtinen, S. J., Ouwehand, A. C., Reinikainen, J. P., Korpela, J. M., Sandholm, J., Salminen, S. J. (2006). Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays.. Appl. Environ. Microbiol. 72: 5132-5134 [Abstract] [Full Text]  


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