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Applied and Environmental Microbiology, March 2005, p. 1668-1670, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1668-1670.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Optical Brighteners Do Not Influence Covert Baculovirus Infection of Spodoptera frugiperda

Ana-Mabel Martínez,1,2 Trevor Williams,1 Miguel López-Ferber,3 and Primitivo Caballero1*

Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona, Spain,1 Instituto de Investigaciones Agropecuarias y Forestales, Tarímbaro, Michoacán, Mexico,2 Laboratoire de Patologie Comparée, UMR 5087, INRA-CNRS-Université de Montpellier II, Saint Christol-Lez-Ales, France3

Received 24 June 2004/ Accepted 18 October 2004

ABSTRACT

Covert infection with Spodoptera frugiperda multiple nucleopolyhedrovirus, detected by reverse transcription-PCR of virus gene transcripts (ie-0 and polh), was not significantly affected by the presence of an optical brightener (Tinopal UNPA-GX), indicating no change in virus virulence. Detection of the covert infection was dependent on insect life stage and the viral mRNA used for diagnosis.

* Corresponding author. Mailing address: Depto. Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain. Phone: (34) 948 169 129. Fax: (34) 948 169 663. E-mail: pcm92{at}unavarra.es.

Applied and Environmental Microbiology, March 2005, p. 1668-1670, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1668-1670.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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