This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jacques, M.-A.
Right arrow Articles by Samson, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jacques, M.-A.
Right arrow Articles by Samson, R.
Agricola
Right arrow Articles by Jacques, M.-A.
Right arrow Articles by Samson, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2005, p. 2008-2015, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2008-2015.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Xanthomonas axonopodis pv. phaseoli var. fuscans Is Aggregated in Stable Biofilm Population Sizes in the Phyllosphere of Field-Grown Beans

M.-A. Jacques,* K. Josi, A. Darrasse, and R. Samson

UMR PaVé, Centre INRA, Beaucouzé, France

Received 27 July 2004/ Accepted 5 November 2004

The occurrence of "Xanthomonas axonopodis pv. phaseoli var. fuscans" (proposed name) populations as biofilms on bean leaves was investigated during three field experiments on plots established with naturally contaminated bean seeds. Behavior of aggregated versus solitary populations was determined by quantification of culturable cells in different fractions of the epiphytic population separated by particle size. X. axonopodis pv. phaseoli var. fuscans population dynamic studies confirmed an asymptomatic and epiphytic colonization of the bean phyllosphere. For all years of experiment and cultivars tested, biofilms and solitary components of the populations were always detected. Biofilm population sizes remained stable throughout the growing season (around 105 CFU/g of fresh weight) while solitary population sizes were more abundant and varied with climate. According to enterobacterial repetitive intergenic consensus fingerprinting, aggregated bacterial isolates were not different from solitary isolates. In controlled conditions, application of a hydric stress resulted in a decrease of the solitary populations on the leaf surface while the biofilm fraction remained stable. Suppression of the hydric stress allowed solitary bacterial populations to increase again. Aggregation in biofilms on leaf surfaces provides protection to the bacterial cells against hydric stress.

* Corresponding author. Mailing address: UMR PaVé, Centre INRA, 42, rue George Morel, BP 60057, 49071 Beaucouzé cedex, France. Phone: (33)-241-22-57-07. Fax: (33)-241-22-57-05. E-mail: jacques{at}angers.inra.fr.

Applied and Environmental Microbiology, April 2005, p. 2008-2015, Vol. 71, No. 4
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.4.2008-2015.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Darsonval, A., Darrasse, A., Meyer, D., Demarty, M., Durand, K., Bureau, C., Manceau, C., Jacques, M.-A. (2008). The Type III Secretion System of Xanthomonas fuscans subsp. fuscans Is Involved in the Phyllosphere Colonization Process and in Transmission to Seeds of Susceptible Beans. Appl. Environ. Microbiol. 74: 2669-2678 [Abstract] [Full Text]  
  • Marquez, M. L., Teran, H., Singh, S. P. (2007). Selecting Common Bean with Genes of Different Evolutionary Origins for Resistance to Xanthomonas campestris pv. phaseoli. Crop Sci. 47: 1367-1374 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»