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Applied and Environmental Microbiology, May 2005, p. 2256-2259, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2256-2259.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Deuterium-Resistant Algal Cell Line for D Labeling of Heterotrophs Expresses Enhanced Level of Hsp60 in D2O Medium

Keiko Unno,* Naoko Hagima, Takahiro Kishido, Shoji Okada, and Naoto Oku

Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan

Received 20 August 2004/ Accepted 9 December 2004

Fully deuterated components from autotrophic cell lysate are useful materials for labeling of heterotrophs with deuterium. To facilitate the faster production of deuterated algal lysate, we selected a mutant Chlorella strain that grows faster in heavy water than the wild type. The mutant DR-17 was found to have a higher level of Hsp60 and an elevated level of protein synthesis. We previously isolated a deuterium-resistant yeast cell line that was also found to express elevated level of Hsp70 (K. Unno, T. Kishido, M. Morioka, S. Okada, and N. Oku, Biol. Pharm. Bull. 26:799-802, 2003). This suggests that the overexpression of heat shock proteins is required to compensate for the deuterium isotope effect.

* Corresponding author. Mailing address: Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. Phone: 81-54-264-5700. Fax: 81-54-264-5705. E-mail: unno{at}u-shizuoka-ken.ac.jp.

Applied and Environmental Microbiology, May 2005, p. 2256-2259, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2256-2259.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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