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Applied and Environmental Microbiology, May 2005, p. 2789-2792, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2789-2792.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetically Engineered Wine Yeast Produces a High Concentration of L-Lactic Acid of Extremely High Optical Purity

Satoshi Saitoh,1,{dagger} Nobuhiro Ishida,2,{dagger}* Toru Onishi,1 Kenro Tokuhiro,2 Eiji Nagamori,2 Katsuhiko Kitamoto,3 and Haruo Takahashi2

Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co., Miyoshi-cho, Aichi 470-0201,1 Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192,2 Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan3

Received 26 August 2004/ Accepted 28 November 2004

For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.

* Corresponding author. Mailing address: Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192, Japan. Phone: 81-561-63-5375. Fax: 81-561-63-6498. E-mail: e1168{at}mosk.tytlabs.co.jp.

{dagger} These two authors contributed equally to this work.

Applied and Environmental Microbiology, May 2005, p. 2789-2792, Vol. 71, No. 5
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.5.2789-2792.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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