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Applied and Environmental Microbiology, July 2005, p. 3413-3419, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3413-3419.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Stable Isotope Fractionation of Tetrachloroethene during Reductive Dechlorination by Sulfurospirillum multivorans and Desulfitobacterium sp. Strain PCE-S and Abiotic Reactions with Cyanocobalamin

Ivonne Nijenhuis,1* Janet Andert,1 Kirsten Beck,1 Matthias Kästner,2 Gabriele Diekert,3 and Hans-Hermann Richnow1

Departments of Isotope Biogeochemistry,1 Bioremediation, UFZ-Centre for Environmental Research Leipzig-Halle GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany,2 Institute for Microbiology, Friedrich-Schiller-Universität, Philosophenweg 12, D-07743 Jena, Germany3

Received 27 October 2004/ Accepted 6 January 2005

Carbon stable isotope fractionation of tetrachloroethene (PCE) during reductive dechlorination by whole cells and crude extracts of Sulfurospirillum multivorans and Desulfitobacterium sp. strain PCE-S and the abiotic reaction with cyanocobalamin (vitamin B12) was studied. Fractionation was largest during the reaction with cyanocobalamin with {alpha}C = 1.0132. Stable isotope fractionation was lower but still in a similar order of magnitude for Desulfitobacterium sp. PCE-S ({alpha}C = 1.0052 to 1.0098). The isotope fractionation of PCE during dehalogenation by S. multivorans was lower by 1 order of magnitude ({alpha}C = 1.00042 to 1.0017). Additionally, an increase in isotope fractionation was observed with a decrease in cell integrity for both strains. For Desulfitobacterium sp. strain PCE-S, the carbon stable isotope fractionation factors were 1.0052 and 1.0089 for growing cells and crude extracts, respectively. For S. multivorans, {alpha}C values were 1.00042, 1.00097, and 1.0017 for growing cells, crude extracts, and the purified PCE reductive dehalogenase, respectively. For the field application of stable isotope fractionation, care is needed as fractionation may vary by more than an order of magnitude depending on the bacteria present, responsible for degradation.

* Corresponding author. Mailing address: Department of Isotope Biogeochemistry, UFZ-Centre for Environmental Research Leipzig-Halle GmbH, Permoserstrasse 15, D-04318 Leipzig, Germany. Phone: 49 (0)341 235-2351. Fax: 49 (0)341 235-2492. E-mail: ivonne.nijenhuis{at}ufz.de.

Applied and Environmental Microbiology, July 2005, p. 3413-3419, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3413-3419.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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