This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hames, C.
Right arrow Articles by Stülke, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hames, C.
Right arrow Articles by Stülke, J.
Agricola
Right arrow Articles by Hames, C.
Right arrow Articles by Stülke, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2005, p. 4097-4100, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4097-4100.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Multiple-Mutation Reaction: a Method for Simultaneous Introduction of Multiple Mutations into the glpK Gene of Mycoplasma pneumoniae

Claudine Hames, Sven Halbedel, Oliver Schilling, and Jörg Stülke*

Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany

Received 19 November 2004/ Accepted 4 January 2005

In Mycoplasma pneumoniae, the UGA opal codon specifies tryptophan rather than a translation stop site. This often makes it difficult to express Mycoplasma proteins in E. coli isolates. In this work, we developed a strategy for the one-step introduction of several mutations. This method, the multiple-mutation reaction, is used to simultaneously replace nine opal codons in the M. pneumoniae glpK gene.

* Corresponding author. Mailing address: Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany. Phone: 49-551-393781. Fax: 49-551-393808. E-mail: jstuelk{at}gwdg.de.

Applied and Environmental Microbiology, July 2005, p. 4097-4100, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4097-4100.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schurwanz, N., Jacobs, E., Dumke, R. (2009). Strategy To Create Chimeric Proteins Derived from Functional Adhesin Regions of Mycoplasma pneumoniae for Vaccine Development. Infect. Immun. 77: 5007-5015 [Abstract] [Full Text]  
  • Hamsten, C., Neiman, M., Schwenk, J. M., Hamsten, M., March, J. B., Persson, A. (2009). Recombinant Surface Proteomics as a Tool to Analyze Humoral Immune Responses in Bovines Infected by Mycoplasma mycoides Subsp. mycoides Small Colony Type. Mol. Cell. Proteomics 8: 2544-2554 [Abstract] [Full Text]  
  • Hames, C., Halbedel, S., Hoppert, M., Frey, J., Stulke, J. (2009). Glycerol Metabolism Is Important for Cytotoxicity of Mycoplasma pneumoniae. J. Bacteriol. 191: 747-753 [Abstract] [Full Text]  
  • Hidalgo, A., Schliessmann, A., Molina, R., Hermoso, J., Bornscheuer, U. T. (2008). A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution. Protein Eng Des Sel 21: 567-576 [Abstract] [Full Text]  
  • Schmidl, S. R., Hames, C., Stulke, J. (2007). Expression of Mycoplasma Proteins Carrying an Affinity Tag in M. pneumoniae Allows Rapid Purification and Circumvents Problems Related to the Aberrant Genetic Code. Appl. Environ. Microbiol. 73: 7799-7801 [Abstract] [Full Text]  
  • Schilling, O., Herzberg, C., Hertrich, T., Vorsmann, H., Jessen, D., Hubner, S., Titgemeyer, F., Stulke, J. (2006). Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucleic Acids Res 34: 6102-6115 [Abstract] [Full Text]  
  • Halbedel, S., Stulke, J. (2006). Probing In Vivo Promoter Activities in Mycoplasma pneumoniae: a System for Generation of Single-Copy Reporter Constructs. Appl. Environ. Microbiol. 72: 1696-1699 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»