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Applied and Environmental Microbiology, July 2005, p. 4101-4103, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4101-4103.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800

Xiao-Zhou Zhang, Zhong-Li Cui, Qing Hong, and Shun-Peng Li*

Department of Microbiology, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, People's Republic of China

Received 26 September 2004/ Accepted 13 January 2005

The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.

* Corresponding author. Mailing address: Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. Phone and fax: 86-25-84396314. E-mail: lsp{at}njau.edu.cn.

Applied and Environmental Microbiology, July 2005, p. 4101-4103, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.4101-4103.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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