This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koort, J.
Right arrow Articles by Björkroth, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koort, J.
Right arrow Articles by Björkroth, J.
Agricola
Right arrow Articles by Koort, J.
Right arrow Articles by Björkroth, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2005, p. 4400-4406, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4400-4406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Lactobacillus oligofermentans sp. nov., Associated with Spoilage of Modified-Atmosphere-Packaged Poultry Products

Joanna Koort,1* Anna Murros,1 Tom Coenye,2 Susanna Eerola,3 Peter Vandamme,2 Antti Sukura,4 and Johanna Björkroth1

Department of Food and Environmental Hygiene, University of Helsinki, Helsinki, Finland,1 Laboratory of Microbiology, University of Ghent, Ghent, Belgium,2 National Veterinary and Food Research Institute, Helsinki, Finland,3 Department of Basic Veterinary Sciences, University of Helsinki, Helsinki, Finland4

Received 11 October 2004/ Accepted 21 February 2005

Unidentified lactic acid bacterium (LAB) isolates which had mainly been detected in spoiled, marinated, modified atmosphere packaged (MAP) broiler meat products during two previous studies, were identified and analyzed for their phenotypic properties and the capability to produce biogenic amines. To establish the taxonomic position of these isolates, 16S rRNA gene sequence analysis, numerical analysis of ribopatterns, and DNA-DNA hybridization experiments were done. Unexpectedly for a meat-spoilage-associated LAB, the strains utilized glucose very weakly. According to the API 50 CHL test, arabinose and xylose were the only carbohydrates strongly fermented. None of the six strains tested for production of histamine, tyramine, tryptamine, phenylethylamine, putrescine, and cadaverine were able to produce these main meat-associated biogenic amines in vitro. The polyphasic taxonomy approach showed that these strains represent a new Lactobacillus species. The six isolates sequenced for the 16S rRNA encoding genes shared the highest similarity (95.0 to 96.3%) with the sequence of the Lactobacillus durianis type strain. In the phylogenetic tree, these isolates formed a distinct cluster within the Lactobacillus reuteri group, which also includes L. durianis. Numerical analyses of HindIII-EcoRI ribotypes placed all isolates together in a cluster with seven subclusters well separated from the L. reuteri group reference strains. The DNA-DNA hybridization levels between Lactobacillus sp. nov. isolates varied from 67 to 96%, and low hybridization levels (3 to 15%) were obtained with the L. durianis type strain confirming that these isolates belong to the same species different from L. durianis. The name Lactobacillus oligofermentans sp. nov. is proposed, with strain LMG 22743T (also known as DSM 15707T or AMKR18T) as the type strain.

* Corresponding author. Mailing address: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 66, FIN-00014 University of Helsinki, Helsinki, Finland. Phone: 358-9-19157119. E-mail: joanna.koort{at}helsinki.fi.

Applied and Environmental Microbiology, August 2005, p. 4400-4406, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4400-4406.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pitkala, A., Koort, J., Bjorkroth, J. (2008). Identification and Antimicrobial Resistance of Streptococcus uberis and Streptococcus parauberis Isolated from Bovine Milk Samples. J DAIRY SCI 91: 4075-4081 [Abstract] [Full Text]  
  • Vihavainen, E., Lundstrom, H.-S., Susiluoto, T., Koort, J., Paulin, L., Auvinen, P., Bjorkroth, K. J. (2007). Role of Broiler Carcasses and Processing Plant Air in Contamination of Modified-Atmosphere-Packaged Broiler Products with Psychrotrophic Lactic Acid Bacteria. Appl. Environ. Microbiol. 73: 1136-1145 [Abstract] [Full Text]  
  • (2005). Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 55: 2235-2238 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»