This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Veneault-Fourrey, C.
Right arrow Articles by Langin, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Veneault-Fourrey, C.
Right arrow Articles by Langin, T.
Agricola
Right arrow Articles by Veneault-Fourrey, C.
Right arrow Articles by Langin, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2005, p. 4761-4770, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4761-4770.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nonpathogenic Strains of Colletotrichum lindemuthianum Trigger Progressive Bean Defense Responses during Appressorium-Mediated Penetration

Claire Veneault-Fourrey,* Richard Laugé, and Thierry Langin

UMR CNRS/UPS 8618, Laboratoire de Phytopathologie Moléculaire, Institut de Biotechnologie des Plantes, Bât. 630, Université Paris XI, 91405 Orsay cedex, France

Received 16 November 2004/ Accepted 17 February 2005

The fungal bean pathogen Colletotrichum lindemuthianum differentiates appressoria in order to penetrate bean tissues. We showed that appressorium development in C. lindemuthianum can be divided into three stages, and we obtained three nonpathogenic strains, including one strain blocked at each developmental stage. H18 was blocked at the appressorium differentiation stage; i.e., no genuine appressoria were formed. H191 was blocked at the appressorium maturation stage; i.e., appressoria exhibited a pigmentation defect and developed only partial internal turgor pressure. H290 was impaired in appressorium function; i.e., appressoria failed to penetrate into bean tissues. Furthermore, these strains could be further discriminated according to the bean defense responses that they induced. Surprisingly, appressorium maturation, but not appressorium function, was sufficient to induce most plant defense responses tested (superoxide ion production and strong induction of pathogenesis-related proteins). However, appressorium function (i.e., entry into the first host cell) was necessary for avirulence-mediated recognition of the fungus.

* Corresponding author. Present address: School of Biological and Chemical Sciences, University of Exeter, Washington Singer Laboratories, Exeter EX4 4QG, United Kingdom. Phone: 44 1392 264689. Fax: 44 1392 264668. E-mail: C.Veneault-Fourrey{at}exeter.ac.uk.

Applied and Environmental Microbiology, August 2005, p. 4761-4770, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4761-4770.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»