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Applied and Environmental Microbiology, September 2005, p. 4960-4965, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.4960-4965.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Application of Fluorescent Amplified Fragment Length Polymorphism for Comparison of Human and Animal Isolates of Yersinia enterocolitica

Catherine Fearnley,1 Stephen L. W. On,2 Branko Kokotovic,2 Georgina Manning,1 Tom Cheasty,3 and Diane G. Newell1*

Department of Food and Environmental Safety, Veterinary Laboratories Agency, New Haw, Surrey,1 Health Protection Agency, Colindale, London, United Kingdom,3 Danish Institute for Food and Veterinary Research, Copenhagen, Denmark2

Received 24 September 2004/ Accepted 18 January 2005

An amplified fragment length polymorphism (AFLP) method, developed to genotype Yersinia enterocolitica, has been used to investigate 70 representative strains isolated from humans, pigs, sheep, and cattle in the United Kingdom. AFLP primarily distinguished Y. enterocolitica strains according to their biotype, with strains dividing into two distinct clusters: cluster A, comprising largely the putatively pathogenic biotypes (BT2 to -4), and cluster B, comprising the putatively nonpathogenic biotype 1A strains and a single BT1B isolate. Within these two clusters, subclusters formed largely on the basis of serotype. However, AFLP profiles also allowed differentiation of strains within these serotype-related subclusters, indicating the high discriminatory power of the technique for Y. enterocolitica. Investigation of the relationship between strain AFLP profile and host confirmed that pigs are, and provides further proof that sheep may be, potential sources of human infection with putatively pathogenic strains. However, the results suggest that some strains causing human disease do not come from veterinary sources identifiable at this time. The distribution of some BT1A isolates within cluster A raises questions about the relationship between virulence potential and biotype.

* Corresponding author. Mailing address: Department of Food and Environmental Safety, Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 1932 357547. Fax: 44 1932 357595. E-mail: d.newell{at}vla.defra.gsi.gov.uk.

Applied and Environmental Microbiology, September 2005, p. 4960-4965, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.4960-4965.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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