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Applied and Environmental Microbiology, September 2005, p. 5341-5347, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5341-5347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Dependence of Arbuscular-Mycorrhizal Fungi on Their Plant Host for Palmitic Acid Synthesis

Martin Trépanier,1* Guillaume Bécard,2 Peter Moutoglis,3 Claude Willemot,1 Serge Gagné,3 Tyler J. Avis,1 and Jacques-André Rioux1

Centre de Recherche en Horticulture, Pavillon de l'Envirotron, Université Laval, Québec G1K 7P4, Canada,1 Pôle de Biotechnologie Végétale, UMR 5546 CNRS/Université Toulouse 3, 24 Chemin de Borde-Rouge, F-31326 Castanet-Tolosan, France,2 Premier Tech Biotechnologies, 1 Avenue Premier, Rivière-du-Loup, Québec G5R 6C1, Canada3

Received 3 November 2004/ Accepted 18 April 2005

Lipids are the major form of carbon storage in arbuscular-mycorrhizal fungi. We studied fatty acid synthesis by Glomus intraradices and Gigaspora rosea. [14C]Acetate and [14C]sucrose were incorporated into a synthetic culture medium to test fatty acid synthetic ability in germinating spores (G. intraradices and G. rosea), mycorrhized carrot roots, and extraradical fungal mycelium (G. intraradices). Germinating spores and extraradical hyphae could not synthesize 16-carbon fatty acids but could elongate and desaturate fatty acids already present. The growth stimulation of germinating spores by root exudates did not stimulate fatty acid synthesis. 16-Carbon fatty acids (16:0 and 16:1) were synthesized only by the fungi in the mycorrhized roots. Our data strongly suggest that the fatty acid synthase activity of arbuscular-mycorrhizal fungi is expressed exclusively in the intraradical mycelium and indicate that fatty acid metabolism may play a major role in the obligate biotrophism of arbuscular-mycorrhizal fungi.

* Corresponding author. Mailing address: Centre de Recherche en Horticulture, Pavillon de l'Envirotron, Université Laval, Québec G1K 7P4, Canada. Phone: (418) 656-2131, ext. 4086. Fax: (418) 656-7871. E-mail: Martin.Trepanier{at}FSAA.ulaval.ca.

Applied and Environmental Microbiology, September 2005, p. 5341-5347, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5341-5347.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schaarschmidt, S., Gonzalez, M.-C., Roitsch, T., Strack, D., Sonnewald, U., Hause, B. (2007). Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity. Plant Physiol. 143: 1827-1840 [Abstract] [Full Text]  


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