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Applied and Environmental Microbiology, September 2005, p. 5572-5576, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5572-5576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genome-Wide Transcriptional Variation within and between Steady States for Continuous Growth of the Hyperthermophile Thermotoga Maritima

Keith R. Shockley,1,{dagger} Kevin L. Scott,2 Marybeth A. Pysz,1,{ddagger} Shannon B. Conners,1 Matthew R. Johnson,1 Clemente I. Montero,1 Russell D. Wolfinger,2 and Robert M. Kelly1*

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905,1 SAS Institute Inc., 100 SAS Campus Drive, Cary, North Carolina 275132

Received 28 December 2004/ Accepted 4 April 2005

Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80°C/0.25 h–1, 80°C/0.17 h–1, and 85°C/0.25 h–1) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.

* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905. Phone: (919) 515-6396. Fax: (919) 515-3465. E-mail: rmkelly{at}eos.ncsu.edu.

{dagger} Present address: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609.

{ddagger} Present address: Roswell Park Cancer Institute, Department of Pharmacology and Therapeutics, Elm and Carlton Streets, Buffalo, NY 14263.

Applied and Environmental Microbiology, September 2005, p. 5572-5576, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5572-5576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Montero, C. I., Johnson, M. R., Chou, C.-J., Conners, S. B., Geouge, S. G., Tachdjian, S., Nichols, J. D., Kelly, R. M. (2007). Responses of Wild-Type and Resistant Strains of the Hyperthermophilic Bacterium Thermotoga maritima to Chloramphenicol Challenge. Appl. Environ. Microbiol. 73: 5058-5065 [Abstract] [Full Text]  
  • Montero, C. I., Lewis, D. L., Johnson, M. R., Conners, S. B., Nance, E. A., Nichols, J. D., Kelly, R. M. (2006). Colocation of Genes Encoding a tRNA-mRNA Hybrid and a Putative Signaling Peptide on Complementary Strands in the Genome of the Hyperthermophilic Bacterium Thermotoga maritima.. J. Bacteriol. 188: 6802-6807 [Abstract] [Full Text]  
  • Nanavati, D. M., Thirangoon, K., Noll, K. M. (2006). Several Archaeal Homologs of Putative Oligopeptide-Binding Proteins Encoded by Thermotoga maritima Bind Sugars. Appl. Environ. Microbiol. 72: 1336-1345 [Abstract] [Full Text]  


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