This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shan, X. C.
Right arrow Articles by Griffiths, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shan, X. C.
Right arrow Articles by Griffiths, M. W.
Agricola
Right arrow Articles by Shan, X. C.
Right arrow Articles by Griffiths, M. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2005, p. 5624-5626, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5624-5626.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid and Quantitative Detection of Hepatitis A Virus from Green Onion and Strawberry Rinses by Use of Real-Time Reverse Transcription-PCR

X. C. Shan, P. Wolffs, and M. W. Griffiths*

Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada

Received 25 October 2004/ Accepted 23 March 2005

In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.

* Corresponding author. Mailing address: Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Phone: (519) 824-4120. Fax: (519) 824-6631. E-mail: mgriffit{at}uoguelph.ca.

Applied and Environmental Microbiology, September 2005, p. 5624-5626, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5624-5626.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Butot, S., Putallaz, T., Sanchez, G. (2007). Procedure for Rapid Concentration and Detection of Enteric Viruses from Berries and Vegetables. Appl. Environ. Microbiol. 73: 186-192 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»