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Applied and Environmental Microbiology, September 2005, p. 5637-5641, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5637-5641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nitrogenase Switch-Off by Ammonium Ions in Azospirillum brasilense Requires the GlnB Nitrogen Signal-Transducing Protein

Giseli Klassen,2 Emanuel M. Souza,1* M. Geoffrey Yates,1 Liu Un Rigo,1 Roberta M. Costa,2 Juliana Inaba,1 and Fábio O. Pedrosa1*

Departamento de Bioquímica e Biologia Molecular,1 Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, Brasil2

Received 3 November 2004/ Accepted 21 March 2005

Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense.

* Corresponding author. Mailing address: Universidade Federal do Paraná, Departamento de Bioquímica e Biologia Molecular, Caixa Postal 19046 CEP-81531-990, Curitiba, Paraná, Brasil. Phone: 55 41 366 4398. Fax: 55 41 266 2042. E-mail for F. O. Pedrosa: fpedrosa{at}ufpr.br. E-mail for E. M. Souza: souzaem{at}ufpr.br.

Applied and Environmental Microbiology, September 2005, p. 5637-5641, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5637-5641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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