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Applied and Environmental Microbiology, September 2005, p. 5642-5645, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5642-5645.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Presence of Two Different Active nirS Nitrite Reductase Genes in a Denitrifying Thauera sp. from a High-Nitrate-Removal-Rate Reactor

Claudia Etchebehere1* and James Tiedje2

Microbiology Department, Faculty of Sciences and Faculty of Chemistry, Universidad de la República, Montevideo, Uruguay,1 Center for Microbial Ecology, Michigan State University, East Lansing, Michigan2

Received 9 December 2004/ Accepted 5 April 2005

The nirS nitrite reductase genes were studied in two strains (strains 27 and 28) isolated from two denitrifying reactors and characterized as Thauera according to their 16S rRNA gene sequences. Strain 28 contains a single nirS sequence, which is related to the nirS of Thauera mechernichensis, and strain 27 contains two nirS sequences; one is similar to the nirS sequence from Thauera mechernichensis (gene 2), but the second one (gene 8) is from a separate clade with nirS from Pseudomonas stutzeri, Azoarcus species, Alcaligenes faecalis, and other Thauera species. Both genes were expressed, but gene 8 was constitutively expressed while gene 2 was positively regulated by nitrate.

* Corresponding author. Mailing address: Cátedra de Microbiología, Facultad de Química, UDELAR, Gral. Flores 2124, CC 1157, Montevideo, Uruguay. Phone: 5982 9244209. Fax: 5982 9244209. E-mail: cetchebe{at}fq.edu.uy.

Applied and Environmental Microbiology, September 2005, p. 5642-5645, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5642-5645.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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