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Applied and Environmental Microbiology, September 2005, p. 5654-5658, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5654-5658.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Loci of Major Antigenic Protein Genes of Edwardsiella tarda

Noel Verjan, Ikuo Hirono, and Takashi Aoki*

Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan

Received 14 December 2004/ Accepted 24 March 2005

Seven antigenic proteins of Edwardsiella tarda were identified by using a rabbit polyclonal antiserum. Four of these proteins also reacted with a Japanese flounder antiserum. The amino acid sequences had identity to lipoproteins, periplasmic proteins, and exported and secreted proteins with roles in transport of metabolites across the cell membrane, stress response, and motility. These genes and their products are useful for developing DNA or recombinant subunit vaccines to control edwardsiellosis.

* Corresponding author. Mailing address: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan. Phone: 81-3-5463-0556. Fax: 81-3-5463-0690. E-mail: aoki{at}s.kaiyodai.ac.jp.

Applied and Environmental Microbiology, September 2005, p. 5654-5658, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5654-5658.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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