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Applied and Environmental Microbiology, January 2006, p. 112-116, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.112-116.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Biotransformation of 1,2,3-Tri- and 1,2,3,4,7,8-Hexachlorodibenzo-p- Dioxin by Sphingomonas wittichii Strain RW1

In-Hyun Nam,1 Young-Mo Kim,1 Stefan Schmidt,2 and Yoon-Seok Chang1*

School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea,1 Abteilung Mikrobiologie, Biozentrum Klein Flottbek, Universität Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany2

Received 23 July 2005/ Accepted 27 September 2005

Sphingomonas wittichii RW1 is able to catabolize 1,2,3,4-tetrachlorodibenzo-p-dioxin (H. B. Hong, Y. S. Chang, I. H. Nam, P. Fortnagel, and S. Schmidt, Appl. Environ. Microbiol. 68:2584-2588, 2002). Here we demonstrate the aerobic bacterial catabolism of the ubiquitous toxic diaryl ether pollutant 1,2,3,4,7,8-hexachlorodibenzo-p-dioxin by this strain. The products of this biotransformation were identified as tetrachlorocatechol and 2-methoxy-3,4,5,6-tetrachlorophenol by comparing mass spectra recorded before and after n-butylboronate and N,O-bis(trimethylsilyl)-trifluoroacetamide derivatization with those of authentic compounds. Additional experiments showed that the less-chlorinated 1,2,3,7,8-pentachlorodibenzo-p-dioxin was not transformed by the strain RW1. The importance of substitution patterns for the degradability of individual congeners was illustrated by the fact that the 1,2,3-trichlorodibenzo-p-dioxin was catabolized to yield 3,4,5-trichlorocatechol, whereas the 2,3,7-trichlorodibenzo-p-dioxin was not attacked.

* Corresponding author. Mailing address: School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. Phone: 82-54-279-2281. Fax: 82-54-279-8299. E-mail: yschang{at}postech.ac.kr

Applied and Environmental Microbiology, January 2006, p. 112-116, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.112-116.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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