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Applied and Environmental Microbiology, January 2006, p. 497-505, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.497-505.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Putative ABC Transporter Responsible for Acetic Acid Resistance in Acetobacter aceti

Shigeru Nakano,1* Masahiro Fukaya,1 and Sueharu Horinouchi2

Central Research Institute, Mizkan Group Co., Ltd., Handa-shi, Aichi 475-8585,1 Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan2

Received 22 July 2005/ Accepted 18 October 2005

Two-dimensional gel electrophoretic analysis of the membrane fraction of Acetobacter aceti revealed the presence of several proteins that were produced in response to acetic acid. A 60-kDa protein, named AatA, which was mostly induced by acetic acid, was prepared; aatA was cloned on the basis of its NH2-terminal amino acid sequence. AatA, consisting of 591 amino acids and containing ATP-binding cassette (ABC) sequences and ABC signature sequences, belonged to the ABC transporter superfamily. The aatA mutation with an insertion of the neomycin resistance gene within the aatA coding region showed reduced resistance to acetic acid, formic acid, propionic acid, and lactic acid, whereas the aatA mutation exerted no effects on resistance to various drugs, growth at low pH (adjusted with HCl), assimilation of acetic acid, or resistance to citric acid. Introduction of plasmid pABC101 containing aatA under the control of the Escherichia coli lac promoter into the aatA mutant restored the defect in acetic acid resistance. In addition, pABC101 conferred acetic acid resistance on E. coli. These findings showed that AatA was a putative ABC transporter conferring acetic acid resistance on the host cell. Southern blot analysis and subsequent nucleotide sequencing predicted the presence of aatA orthologues in a variety of acetic acid bacteria belonging to the genera Acetobacter and Gluconacetobacter. The fermentation with A. aceti containing aatA on a multicopy plasmid resulted in an increase in the final yield of acetic acid.

* Corresponding author. Mailing address: Central Research Institute, Mizkan Group Co., Ltd., Handa-shi, Aichi 475-8585, Japan. Phone: 81 (569) 24-3331. Fax: 81 (569) 24-5024. E-mail: snakano1{at}mizkan.co.jp

Applied and Environmental Microbiology, January 2006, p. 497-505, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.497-505.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mullins, E. A., Francois, J. A., Kappock, T. J. (2008). A Specialized Citric Acid Cycle Requiring Succinyl-Coenzyme A (CoA):Acetate CoA-Transferase (AarC) Confers Acetic Acid Resistance on the Acidophile Acetobacter aceti. J. Bacteriol. 190: 4933-4940 [Abstract] [Full Text]  


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