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Applied and Environmental Microbiology, January 2006, p. 880-889, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.880-889.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Surface Display System for Proteins on Non-Genetically Modified Gram-Positive Bacteria

Tjibbe Bosma,1 Rolf Kanninga,1 Jolanda Neef,1 Sandrine A. L. Audouy,1 Maarten L. van Roosmalen,1 Anton Steen,2 Girbe Buist,2 Jan Kok,2 Oscar P. Kuipers,2 George Robillard,1 and Kees Leenhouts1*

BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands,1 Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands2

Received 9 May 2005/ Accepted 21 October 2005

A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable. This system is based on nonliving and non-genetically modified gram-positive bacterial cells, designated gram-positive enhancer matrix (GEM) particles, which are used as substrates to bind externally added heterologous proteins by means of a high-affinity binding domain. This binding domain, the protein anchor (PA), was derived from the Lactococcus lactis peptidoglycan hydrolase AcmA. GEM particles were typically prepared from the innocuous bacterium L. lactis, and various parameters for the optimal preparation of GEM particles and binding of PA fusion proteins were determined. The versatility and flexibility of the display and delivery technology were demonstrated by investigating enzyme immobilization and nasal vaccine applications.

* Corresponding author. Mailing address: BiOMaDe Technology, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Phone: 315 03638146. Fax: 315 03634429. E-mail: Leenhouts{at}biomade.nl

Applied and Environmental Microbiology, January 2006, p. 880-889, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.880-889.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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